FastQCFastQC Report
Sat 31 Dec 2022
SRR1954473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380585
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43341.138773204409002No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG30150.7922014793016016No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC20410.5362796747112997No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC17110.44957105508624884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8270.21729705584823367No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC6470.17000144514366042Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT6180.16238159675236805Illumina PCR Primer Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT5380.1413613253281133No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC5210.13689451765045915No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA4200.11035642497733752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)