FastQCFastQC Report
Sat 31 Dec 2022
SRR1954457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187618
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.6976942510846508No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG10190.5431248600880513No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC7480.39868242919122904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6700.3571085929921436No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC6160.32832670639277683No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT2270.12099052329733821No Hit
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT2100.11192955899753756No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC2060.10979756739758445TruSeq Adapter, Index 20 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)