FastQCFastQC Report
Sat 31 Dec 2022
SRR1954454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280599
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.7690690273308173No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG19300.6878142830159765No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC17470.6225966592895913No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC13390.47719343262092884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8610.3068435739257802No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT3840.13685009568815285No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC3550.12651506242003713TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA3300.11760555098200634No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT3230.11511088777935773No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC2890.10299395222363586No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)