FastQCFastQC Report
Sat 31 Dec 2022
SRR1954447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310860
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.8347809303223316No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG21740.6993501897960497No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC17770.5716399665444252No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC14030.4513285723476806No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9640.3101074438654056No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT4680.15055008685581933Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT4600.1479765810976002No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC4150.13350061120761758No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3530.1135559415814193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)