FastQCFastQC Report
Sat 31 Dec 2022
SRR1954446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277373
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG26220.9452974874987832No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.8230793912889863No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC19040.6864402807771485No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC16420.5919826370987803No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9030.3255543978685741No Hit
GAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT5390.19432316772000158No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT4690.16908639269142994No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTC4130.14889697266857266No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC4040.14565224445061345TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTA3630.13087070479102147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)