FastQCFastQC Report
Sat 31 Dec 2022
SRR1954439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324134
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.7499984574281008No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG18140.5596450850574145No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC14380.44364367823184236RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC12610.389036632997464No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10250.3162272393516262No Hit
GAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT3930.12124615128311131TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC3250.10026717345295465No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)