FastQCFastQC Report
Sat 31 Dec 2022
SRR1954435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517913
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG37910.7319762199442763No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.7223220888450377No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC32980.636786487305783No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC23940.46223979703154777No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT9950.19211720887484965No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC8660.16720955063881388No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC7520.14519813173254967No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT6740.13013768721773733No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5760.11121559026322954No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5240.10117529392002131No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)