FastQCFastQC Report
Sat 31 Dec 2022
SRR1954431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470502
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50241.0677956735571794No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG37710.8014843720111711No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC29440.6257146622118503No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC18220.38724596282268725No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC9090.19319790351581928TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT7940.16875592452316887No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC6940.14750202974695115No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT6480.137725238149891No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4890.10393154545570477No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4710.10010584439598556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)