FastQCFastQC Report
Sat 31 Dec 2022
SRR1954428.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954428.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417718
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG48921.1711250173562069No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC42261.0116873105779496TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC41150.9851143594482402No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.8939045001651833No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT11850.28368420800635835TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC9940.23795958038676812TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC9810.23484743295716248No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT9290.22239884323874004No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8080.1934319325477954No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA7820.18720763768858417No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT5500.13166777586793005No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT5140.12304952144748371No Hit
GAATCTATCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC4770.11419187107091387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)