FastQCFastQC Report
Sat 31 Dec 2022
SRR1954413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355438
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.9996117466337308No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCG27920.7855097091475869No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTC20920.5885695958226188No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGC17000.4782831323606367No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6930.19497071219171838No Hit
GAACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCT6080.17105655557368654No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5600.15755209065997444No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCT5380.15136254424118975No Hit
GCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC5180.14573568386047636TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTC5180.14573568386047636No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTT4370.12294689931858721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)