FastQCFastQC Report
Thu 19 Jan 2023
SRR1954409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306936
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG20820.6783173039330676No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19960.6502984335496651No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC15480.5043396669012432No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC13970.4551437433210832No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12880.4196314541142127No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT4190.1365105429144838No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC3460.11272708317043292TruSeq Adapter, Index 13 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT3390.11044647744155134No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC3390.11044647744155134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG351.2080454E-745.00000412
ACCGCAG253.884101E-545.0000041
ATGTCGG253.884101E-545.0000042
AACGGAG253.884101E-545.0000042
CGGGAAC351.2080454E-745.0000046
TAGTGCG253.884101E-545.0000041
TTCGCTC351.2080454E-745.00000415
AGATGCG253.884101E-545.00000425
GTTTGCG207.025198E-445.01
CGGAATA207.025198E-445.017
CCGGGCT207.025198E-445.05
TAGAATA207.025198E-445.018
TTGTACG207.025198E-445.01
TAAGTGT207.025198E-445.016
AAGCGCG207.025198E-445.01
ATCACGC207.025198E-445.040
TGCATCG207.025198E-445.024
TGGCACA207.025198E-445.036
CCCGTAG207.025198E-445.027
GCCGAGT207.025198E-445.09