FastQCFastQC Report
Sat 31 Dec 2022
SRR1954406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458051
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.9263160652416434No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC30580.6676112485290939No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG30160.6584419638861175No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC26340.5750451368952365No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCT7420.1619906953592504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6600.14408875867534401No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC5370.11723585364948445Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTC5280.11527100694027521No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTA5190.11330616023106597No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCT4840.10566508969525228No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)