FastQCFastQC Report
Sat 31 Dec 2022
SRR1954404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325866
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.821503317314479No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG12660.38850324980206585No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC8620.2645259094228916RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC7910.24273781247506643No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3970.12182921814488164No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3630.11139548157831747No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)