FastQCFastQC Report
Sat 31 Dec 2022
SRR1954403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407207
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG61021.49850076251145No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC44201.085443030203312RNA PCR Primer, Index 10 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC35970.8833345202808399No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.856321232198857No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC11610.28511297693801924RNA PCR Primer, Index 35 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT11290.2772545658596242TruSeq Adapter, Index 10 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT10780.26473022320343215No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC9600.23575233235185053No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8200.20137178388387234No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTA7510.18442708499608307No Hit
GAATCTATCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC5030.12352439913852167TruSeq Adapter, Index 10 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT4690.11517483736772699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)