FastQCFastQC Report
Sat 31 Dec 2022
SRR1954401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323493
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG61871.912560704559295No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC44111.363553461744149No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC35721.104196999625958No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.7666317354625912No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT12410.38362499343107886No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC10160.3140717109798357No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT9890.3057253170856865No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC9200.2843956438006387TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA7410.22906214353942744No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6060.18733017406868155No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT5370.16600050078363363No Hit
GAATCTATCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC4470.13817918780313637No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGACCTATCGT3330.10293885802783986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)