FastQCFastQC Report
Sat 31 Dec 2022
SRR1954400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429793
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44491.0351494789352083No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG33230.7731628946958187No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC22910.533047304167355No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC15000.3490052187913717No Hit
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT7800.18148271377151326RNA PCR Primer, Index 23 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7340.1707798870619112No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6690.15565632758095174No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC6150.14309213970446238No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT5110.11889444453492728No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC4610.10726093724188156RNA PCR Primer, Index 23 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)