FastQCFastQC Report
Sat 31 Dec 2022
SRR1954393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326939
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.7854676254591835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG19290.5900183214605783No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC13640.417203209161342No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC12130.3710172233964134No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7760.23735314538797758No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC4240.1296878010882764TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT3950.12081764488176693No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)