FastQCFastQC Report
Sat 31 Dec 2022
SRR1954391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327914
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG45171.3774953188945882No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC36221.1045579023768426No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC30160.9197533499637099No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.8282659477789908No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC10060.3067877553260916No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT9710.2961142250712077No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8730.2662283403575328No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC7670.23390279158559865No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT7370.22475405136712676No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT7130.21743505919234923No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA5910.18023018230389676No Hit
GAATCTATCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC4000.12198320291295889No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3580.10917496660709819No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT3410.10399068048329745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)