FastQCFastQC Report
Sat 31 Dec 2022
SRR1954389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350107
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.8837298311659006No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG22400.6398044026540458No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC14700.4198716392417175No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC13790.39387958538389695RNA PCR Primer, Index 40 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8900.25420799926879495No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC4170.11910644460122191Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT3760.10739573901692911TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC3640.10396821543128243No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)