FastQCFastQC Report
Sat 31 Dec 2022
SRR1954385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426380
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443103.1064653177975012No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG170301.1939314909070515No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC132230.9270320671910711TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC90850.6369270460887No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51190.35888052272185533No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT42380.29711577559977004No Hit
GAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT40100.2811312553457003No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC35480.2487415695677169TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC34940.24495576213912143No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC22120.1550778894824661TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCTCTGCT21600.15143229714381862No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTA17000.11918282645578317No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT15120.10600260800067303Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)