FastQCFastQC Report
Sat 31 Dec 2022
SRR1954382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902564
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369334.092008987728294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31210.3457926529309833No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC11430.1266392189362749RNA PCR Primer, Index 10 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG9740.10791478499031647No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC9230.1022642161663882RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)