FastQCFastQC Report
Sat 31 Dec 2022
SRR1954381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652225
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233133.5743800068994593No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG67861.0404384989842463No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC53950.8271685384644869RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC40470.6204913948407375No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT14850.22768216489708307TruSeq Adapter, Index 3 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT14750.22614895166545287No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC12660.19410479512438192TruSeq Adapter, Index 18 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC11830.18137912530185135No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10030.15378128713250797No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGCAACT8220.12603012764000154No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTA6710.10287860784238569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)