FastQCFastQC Report
Sat 31 Dec 2022
SRR1954379.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954379.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875792
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314903.595602608838629No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC58200.6645413522845607TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG53840.6147578420446864No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC31810.36321409649779857No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT18330.20929627126075595Illumina PCR Primer Index 3 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17380.1984489467818843No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC13000.148437071816139No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT12510.14284213603229992No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11140.12719915231013756TruSeq Adapter, Index 14 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)