FastQCFastQC Report
Sat 31 Dec 2022
SRR1954378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572855
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT853593.317676277909171No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC121640.4727821816620058No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG120580.4686622448602817No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC61740.23996688503627292No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT54370.2113216640657946TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC34890.13560810850203375Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC28500.11077188570673435No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT26170.10171579820860484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)