FastQCFastQC Report
Sat 31 Dec 2022
SRR1954377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663662
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367915.543635163682718No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG47370.7137669476329819No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC39100.5891553230409455No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC32180.4848853784004508No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24460.36856110489978333No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC13620.2052249488444419No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT11630.17523980580476206No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT10220.15399405118870751No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT7670.11557087794690671No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC7260.10939303440606815No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT6780.10216043708996447Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC6730.10140704153620365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)