FastQCFastQC Report
Sat 31 Dec 2022
SRR1954375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534230
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494763.224809839463444No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG71220.4642068008056158No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC68400.4458262450871121TruSeq Adapter, Index 27 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43560.28392092450284506No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC35210.22949622937890665TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC34250.2232390189215437No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT28420.1852395012481831TruSeq Adapter, Index 27 (100% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT24890.16223121696225468TruSeq Adapter, Index 27 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC23610.1538882696857707TruSeq Adapter, Index 27 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT18250.1189521779654941No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC16410.10695919125554838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)