FastQCFastQC Report
Sat 31 Dec 2022
SRR1954372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160824
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419263.6117447606183193No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG103540.8919526129714755No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC87580.7544640703500272No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC68520.5902703596755409No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53930.4645837784194675No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC25240.21743175537376896TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT22790.1963260580415291No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC21070.18150899705726276No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT20520.17677098337043343TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT13290.1144876398144766No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT12900.11112795738199761TruSeq Adapter, Index 20 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)