FastQCFastQC Report
Thu 19 Jan 2023
SRR1954371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406803
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427053.0356062647008857No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG66320.47142350421487583No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC50040.3557001229027803No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38760.27551832061774106No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC32750.2327973426272193No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC17970.12773643502324064RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT15040.10690906971338561RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG302.1658489E-645.0000041
GGTCCGC302.1658489E-645.00000430
TCTAGCG302.1658489E-645.00000420
GAACGTA302.1658489E-645.00000422
CGTCGAA750.045.00000442
CGGTCCG302.1658489E-645.00000429
CGTCCAT302.1658489E-645.00000425
GCGTCCA302.1658489E-645.00000424
CGTTTCG302.1658489E-645.00000442
CCGCAAT302.1658489E-645.00000433
TCCGGAC207.033827E-445.016
GCGCGGC207.033827E-445.027
GACCGTA207.033827E-445.018
TAGCGGA253.8912534E-545.03
TAGCGCA351.2121927E-745.01
CGCGCCC253.8912534E-545.034
ACTTCGC207.033827E-445.034
ACTTCCG551.8189894E-1245.01
TAACGCG207.033827E-445.01
CCGTAGA207.033827E-445.020