FastQCFastQC Report
Sat 31 Dec 2022
SRR1954366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739772
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145571.9677684475757395No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG29190.39458103307505554No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC27400.3703843887035465Illumina PCR Primer Index 2 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20600.27846417544865176No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC14260.19276209426688223No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC10900.14734269477622836Illumina PCR Primer Index 2 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT8980.12138875221014044Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)