FastQCFastQC Report
Sat 31 Dec 2022
SRR1954363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847916
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417404.922657433047614No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG59090.6968850688039853No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC54940.6479415413790989No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC43060.5078333231121951No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20830.24566112680973112No Hit
GAACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT14970.17655050736157826Illumina PCR Primer Index 8 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC13930.16428514145269107Illumina PCR Primer Index 8 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT12990.15319913765042764No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10620.1252482557234443No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC10420.12288953151019676No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGGAAGCT9260.10920893107336103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)