FastQCFastQC Report
Sat 31 Dec 2022
SRR1954362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080221
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487504.512965402450054No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG121021.1203263035989859No Hit
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC105670.9782257519526096No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC79390.7349422016420715No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC29720.27512888566321153TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC27310.25281863618648404No Hit
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT27260.2523557679400789No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT26580.2460607597889691No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20930.19375664794518901No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC19480.18033346879944012No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT18340.16978007278140306No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT15460.14311886178846736TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT11430.10581168112821358No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC10860.10053498311919505TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)