FastQCFastQC Report
Sat 31 Dec 2022
SRR1954361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913033
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251702.7567459226555884No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG52190.5716113218251695No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC48290.5288965459079792No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC34050.37293285127700754No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31980.3502611625209604No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC12400.13581108240337425TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT12280.13449678160592224No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT10660.11675372084032011No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC10220.11193461791632943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)