FastQCFastQC Report
Sat 31 Dec 2022
SRR1954358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915193
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234912.566780995921079No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26020.2843116151456578No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG19020.2078250161441357No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC18360.2006134225239922No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12000.1311198840026093No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT10650.11636889705231575TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC10120.1105777688422005TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC10070.11003143599218963TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)