FastQCFastQC Report
Sat 31 Dec 2022
SRR1954356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491627
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145922.968103867362858No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC39990.8134215574002241No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG37290.7585018723544475No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC26080.5304834762940197No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13970.28415851855166624No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT11260.22903542726497936No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC9550.19425296006932083No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT7330.14909677458723788No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC6920.14075711870991625No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT5520.11228024498247656No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT5270.10719508895971946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)