FastQCFastQC Report
Sat 31 Dec 2022
SRR1954355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932300
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384204.120991097286281No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC57650.618363187815081No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG52120.5590475168937037No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC37670.40405448889842327No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31430.3371232435911187No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT19940.21387965247238014No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT19190.20583503164217526No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC17910.1921055454252923Illumina Single End Adapter 1 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC14160.15188244127426795No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13150.1410490185562587No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT11780.12635417783975117No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT10570.11337552290035396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)