FastQCFastQC Report
Sat 31 Dec 2022
SRR1954345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674806
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.20079845170315616No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC12510.1853866148196667TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT10470.15515570400974502Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC9640.1428558726508063TruSeq Adapter, Index 23 (95% over 24bp)
GAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT9000.1333716653378897TruSeq Adapter, Index 23 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8710.12907413389922437No Hit
AAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT8280.12270193211085853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)