FastQCFastQC Report
Sat 31 Dec 2022
SRR1954343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983230
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21710.2208028640297794No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.17747627716810918No Hit
GAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT13340.135675274350864No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT12470.12682688689319893TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC11100.11289321928745055TruSeq Adapter, Index 19 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT10510.10689258871271218No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC10350.1052652990653255TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)