FastQCFastQC Report
Sat 31 Dec 2022
SRR1954342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463517
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG10960.23645303192763156No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC10580.22825484286444725No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.18381202846929023No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7730.16676842489056495No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC6490.14001643952648987No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC4790.1033403305596127RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)