FastQCFastQC Report
Sat 31 Dec 2022
SRR1954340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763682
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21050.2756382892355719No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.2359620889323043No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC16070.2104279006183202No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT11760.15399079721664252Illumina Single End Adapter 1 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT9720.12727810790355149No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG9460.12387354946168692No Hit
GAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT8650.1132670404697243No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC7770.10174391958956738No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC7730.10122014136774207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)