FastQCFastQC Report
Sat 31 Dec 2022
SRR1954334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513029
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17370.3385773513777974No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.23643887577505365No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC7120.13878357753655252No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT6550.12767309450342962No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT6290.12260515487428586Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC6290.12260515487428586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5270.1027232378676449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)