FastQCFastQC Report
Sat 31 Dec 2022
SRR1954327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968825
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.22563414445333266No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT10910.11261063659587646TruSeq Adapter, Index 22 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10650.10992697339560807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)