FastQCFastQC Report
Sat 31 Dec 2022
SRR1954326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686855
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20450.2977338739617532No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.2364400055324632No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC12950.18854052165304178TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT12090.17601968392164286TruSeq Adapter, Index 20 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC9810.14282490481979457TruSeq Adapter, Index 23 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7650.11137721935488568No Hit
AAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7190.10468002707995137TruSeq Adapter, Index 20 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7000.10191379548813069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)