FastQCFastQC Report
Sat 31 Dec 2022
SRR1954325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865133
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23000.2658550766182772No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.249094647874951No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC19490.22528328014305316No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT17430.2014719124111553No Hit
GAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT15370.17766054467925743No Hit
AAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT14920.17245903231063894No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC12570.14529557883007582No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC10000.11558916374707705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9270.10715115479354041No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC8690.10044698329620995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)