FastQCFastQC Report
Sat 31 Dec 2022
SRR1954319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874949
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.20641203087265655No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC17480.19978307307054471Illumina PCR Primer Index 2 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16380.18721091172171178No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT14320.1636666822866247Illumina PCR Primer Index 2 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC10680.12206425745957764Illumina PCR Primer Index 2 (95% over 24bp)
GAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT10340.1181783166790293Illumina PCR Primer Index 2 (95% over 22bp)
AAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT9410.10754912572047057Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)