FastQCFastQC Report
Sat 31 Dec 2022
SRR1954313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730568
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG37660.5154893179005925No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC30040.4111869121012692No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC29810.4080386767556203No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17710.2424141216149626No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.221197752981242No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC13200.18068133288071747Illumina PCR Primer Index 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT10800.14783018144785975TruSeq Adapter, Index 10 (95% over 24bp)
GAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT9370.12825637038578203No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC8240.1127889532528115RNA PCR Primer, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)