FastQCFastQC Report
Sat 31 Dec 2022
SRR1954312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866113
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.19489373788408673No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14250.164528185121341No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC9420.10876178974337067TruSeq Adapter, Index 13 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT9220.1064526222328957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)