FastQCFastQC Report
Sat 31 Dec 2022
SRR1954310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628213
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20010.3185225393298133No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.1883119260505593No Hit
GAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT8820.14039824072408563TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC6850.10903944999546332TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT6290.10012527598123568Illumina Single End Adapter 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)