FastQCFastQC Report
Sat 31 Dec 2022
SRR1954293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750039
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20120.26825271752535534No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.22812147101684047No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12700.16932452845785353No Hit
GAGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT11040.14719234599800812No Hit
AAGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT10290.13719286597096952No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC9670.1289266291486176No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9020.12026041312518415No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8640.11519400991148461No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT8340.11119421790066918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.10772773149129579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)