FastQCFastQC Report
Sat 31 Dec 2022
SRR1954291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738248
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.20738288488421236No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13330.18056262936032336No Hit
GAGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT12430.168371604122192Illumina PCR Primer Index 9 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC11410.1545551088523098Illumina PCR Primer Index 9 (95% over 23bp)
AAGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT9690.13125670506388099Illumina PCR Primer Index 9 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT9450.12800576500037927RNA PCR Primer, Index 17 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8760.1186593123178119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)