FastQCFastQC Report
Sat 31 Dec 2022
SRR1954279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152348
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21250.18440609954631762No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.1719098744476495No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC17880.1551614616417957No Hit
GAGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT16850.1462231895226095No Hit
AAGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT14830.12869376264808893No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT13870.12036294591564355Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)